Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: Confirmation of specificity of interactions observed in yeast 2-hybrid assay. Panels show yeast transformed with a) psoriasin and RanBPM expression plasmids (left) and RanBPM alone (right), and yeast transformed with b) Psoriasin alone, c) psoriasin and empty prey vector, and d) psoriasin with a control gene not isolated in the primary screen. In panels b-d, plates on left are Histidine + (control) and plates on the right are Histidine – (test). Panel a) shows activation of LacZ reporter gene only occurs in yeast transformed with both expression plasmids (left) and no activation in yeast transformed with RanBPM alone (right). Panel b) shows that psoriasin alone cannot activate the Histidine reporter gene as demonstrated by absence of growth on Histidine – plate. Panels c) and d) show that there is a specific interaction necessary for activation of the reporter gene.

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: Y2H Assay, Transformation Assay, Expressing, Plasmid Preparation, Isolation, Activation Assay

Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: In vitro interaction of psoriasin and RanBPM as determined by co-immunoprecipitation. 35 S-Met labelled proteins were generated. Psoriasin (lane 1) and RanBPM (lane 2) were electrophoresed through a denaturing polyacrylamide gel and detected by autoradiography. Psoriasin binds to and co-immunoprecipitates with RanBPM (lane 4). RanBPM and psoriasin do not bind to protein G-beads on their own, (lane 3 and 5 respectively). RanBPM does not interact with mouse submaxillary gland protein when either RanBPM (lane 6) or mouse submaxillary gland protein (lane 7) is precipitated.

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: In Vitro, Immunoprecipitation, Generated, Autoradiography

Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: Relationship Between RanBPM and Psoriasin Expression and Clinical-Pathological Parameters in Human Breast Cancer

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: Expressing

Journal: Computer aided surgery : official journal of the International Society for Computer Aided Surgery

Article Title: URobotics--Urology Robotics at Johns Hopkins

doi: 10.1002/igs.10021

Figure Lengend Snippet: Teleoperated Karl Storz laparoscopy equipment and the BW-RCM robot with the Lap Driver supporting a tissue retractor. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com]

Article Snippet: [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com ] fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Teleoperated Karl Storz laparoscopy equipment and the BW-RCM robot with the Lap Driver supporting a tissue retractor.

Techniques:

Journal: Pharmaceutics

Article Title: Modulation of Multispecific Transporters by Uncaria tomentosa Extract and Its Major Phytoconstituents

doi: 10.3390/pharmaceutics16111363

Figure Lengend Snippet: Relative transporter-specific transport of the validated SLC transporter substrates in the presence of 100 µM uncarine D, mitraphylline, or isopteropodine. Data are relative to the vehicle control. All data are presented as the mean ± SEM.

Article Snippet: Mitraphylline (purity 99.66%) and uncarine D (purity 97%) were from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany), and isopteropodine (USP reference standard) was obtained from Merck KGaA.

Techniques: Control

Journal: Pharmaceutics

Article Title: Modulation of Multispecific Transporters by Uncaria tomentosa Extract and Its Major Phytoconstituents

doi: 10.3390/pharmaceutics16111363

Figure Lengend Snippet: Relative transporter-specific transport of the validated ABC transporter substrates in the presence of 100 µM uncarine D, mitraphylline, or isopteropodine. Data are relative to the vehicle control. All data are presented as the mean ± SEM.

Article Snippet: Mitraphylline (purity 99.66%) and uncarine D (purity 97%) were from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany), and isopteropodine (USP reference standard) was obtained from Merck KGaA.

Techniques: Control

Journal: Pharmaceutics

Article Title: Modulation of Multispecific Transporters by Uncaria tomentosa Extract and Its Major Phytoconstituents

doi: 10.3390/pharmaceutics16111363

Figure Lengend Snippet: IC 50 values of mitraphylline and isopteropodine determined in transporter-overexpressing cell lines.

Article Snippet: Mitraphylline (purity 99.66%) and uncarine D (purity 97%) were from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany), and isopteropodine (USP reference standard) was obtained from Merck KGaA.

Techniques:

Journal: Pharmaceutics

Article Title: Modulation of Multispecific Transporters by Uncaria tomentosa Extract and Its Major Phytoconstituents

doi: 10.3390/pharmaceutics16111363

Figure Lengend Snippet: IC 50 values of mitraphylline and isopteropodine determined for BCRP and MDR1.

Article Snippet: Mitraphylline (purity 99.66%) and uncarine D (purity 97%) were from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany), and isopteropodine (USP reference standard) was obtained from Merck KGaA.

Techniques: